Bioprinting is a type of 3D printing to manufacture biological. So far, the process has been used to develop organs or organoidssmaller versions of organs that are at least partially functionaland can be used as models for research. Ultimately researchers hope they can bioprint organs that can be used for transplants.
Chicago-based BIOLIFE4D recently bioprinted a 3D miniature human heart, which they say is a big step toward producing a full-sized human heart that could be used for transplant. The company has research facilities at JLABS in Houston.
The miniature heart had all the structures of a full-sized heart, with four internal chambers. The company says it is as close as anyone has gotten to a fully functioning heart via 3D bioprinting.
We are extremely proud of what we have accomplished, from the ability to 3D bioprint human cardiac tissue last summer to a mini heart with full structure now, said Ravi Birla, the companys chief science officer. These milestones are a testament to the hard work of our team and the proprietary process we have developed that enables this type of scientific achievement. We believe we are at the forefront of whole heart bioengineering, a field that has matured quickly over the last year, and well positioned to continue our rapid scientific advancement. Today is an exciting day, but we continue forward earnestly toward the end goal of 3D bioprinting whole human hearts.
3D printing is sometimes called additive manufacturing. It is a way of making three-dimensional solid objects from a digital file. In many ways, the only limitations are the limits on the complexity of the design. 3D printing used in industrial applications typically use carbon fiber as a source material. But bioprinting uses a variety of biological materials, such as single cell suspensions, as the source materials.
In the case of BIOLIFE4Ds heart, they developed a proprietary bioink with a unique composition of different extracellular matrix compounds. These compounds are very similar to the properties of a mammalian heart. The company also developed a novel bioprinting algorithm made up of printing parameters optimized for the whole heart, which it coupled with patient-derived cardiomyocytes. Although the heart is small in size, it has many of the features of a human heart.
BIOLIFE4D isnt the only company working in this specific area. In April, researchers at Tel Aviv University successfully printed the first 3D human heart. The research team used the patients own cells and various biological materials such as collagen and glycoprotein. Their work was published in the journal Advanced Science.
This heart is made from human cells and patient-specific biological materials, stated Tal Dvir, lead researcher. In our process these materials serve as the bioinks, substances made of sugars and proteins that can be used for 3D printing of complex tissue models. People have managed to 3D-print the structure of a heart in the past, but not with cells or with blood vessels. Our results demonstrate the potential of our approach for engineering personalized tissue and organ replacement in the future.
Dvir and his team began by taking biopsies of fatty tissues from the omentum, a fold of visceral peritoneum that hangs from the stomach, in the abdomen of humans and pigs. They then separated the cellular materials from extraneous materials and reprogrammed the cellular materials to become pluripotent stem cells. From these, they were able to develop all three body layers that had the potential to produce any cell or tissue in the body.
They then built an extracellular matrix from collagen and glycoproteins into a hydrogel using the bioprinter. They mixed the cells with the hydrogel, which were then differentiated into cardiac or endothelial cells. This created what theyre calling patient-specific, immune-compatible cardiac patches complete with blood vessels.
From that point, they then created an entirebut smallbioengineered and bioprinted human heart.
Last year, Poietis, a Pessac, France-based company, along with Prometheus, a division of Skeletal Tissue Engineering at Leuven, Belgium, announced they had entered into a two-year Collaborative Research Agreement to develop high-precision 3D Bioprinting of tissue engineered Advanced Therapeutic Medicinal Products (ATMPs) for skeletal regeneration.
Prometheus focuses on tissue-engineered ATMPs with a focus on skeletal regeneration. Poietis is interested in using 3D bioprinting of single cell suspensions into large, patterned tissue structures, especially the laser-assisted bioprinting of multicellular micro-aggregates embedded in bioinks for the formation of layered cellular structures.
What this comes down to is a collaboration to print bone that can be used in transplants or other orthopedic, musculoskeletal or spine-related applications.
Poietis already has a product on the market, Poieskin, a human full thickness skin model produced entirely by 3D bioprinting. It is made up of a dermal compartment composed of primary human fibroblasts embedded in a collagen I matrix overlaid by a stratified epidermis derived from primary human keratinocytes.
In May, researchers with Rice University developed a new approach resulting in exquisitely entangled vascular networks that mimic the bodys natural passageways for blood, air, lymph and other vital fluids. The research was published in the journal Science.
One of the biggest roadblocks to generating functional tissue replacements has been our inability to print the complex vasculature that can supply nutrients to densely populated tissues, stated Jordan Miller of Rice University. Further, our organs actually contain independent vascular networkslike the airways and blood vessels of the lung or the bile ducts and blood vessels in the liver. These interpenetrating networks are physically and biochemically entangled, and the architecture itself is intimately related to tissue function. Ours is the first bioprinting technology that addresses the challenge of multivascularization in a direct and comprehensive way.
Also in May, San Diego-based Organovo entered a collaboration agreement with Melissa Little at the Murdoch Childrens Research Institute (MCRI), The Royal Childrens Hospital, in Melbourne, Australia, and Ton Rabelink at Universiteit Leiden (LUMC), Leiden, Netherlands. The collaboration will focus on expanding the use of 3D bioprinted stem cell-based therapeutic tissues. The goal is to develop treatments for end-stage renal disease.
The collaboration will utilize Organovos bioprinting platform, MCRIs advanced stem cell differentiation technology, and LUMCs cell lines and clinical expertise. The partnership is funded by Stem Cells Australia and CSL Limited.
And in 2018, United Therapeutics and Lung Biotechnology made a collaboration pact with Israeli 3D bioprinting company CollPlant. United paid CollPlant $5 million up front with up to $15 million in milestones to supply bioink to Lung Biotechnology. CollPlants recombinant human collagen (rhCollagen) is grown from tobacco plants engineered with five human genes. The purified collagen can be used as a scaffold for 3D bioprinting solid organs.
BIOLIFE4D has had several breakthroughs in this area. Earlier this year it successfully 3D bioprinted individual heart components, and in June 2018 it successfully 3D bioprinted a cardiac patch out of human cardiac tissue.
The companys 3D bioprinting process gives the researchers the opportunity to reprogram a patients own white blood cells to induced pluripotent stem (iPS) cells, then to force the iPS cells to differentiate into different types of cardiac cells to be used as individual cardiac components and eventually, into a human heart that could be used for transplant.
This is an incredibly exciting time for BIOLIFE4D, and we are so proud of Dr. Birla and the team for this tremendous accomplishment, said Steven Morris, the companys chief executive officer. We began this journey with an end goal of developing a technology that has the potential to save lives, and we are a step closer to that today. We will continue our work until we are able to 3D bioprint full-sized hearts for viable transplant, and change the way heart disease is treated forever.
- UK animal experiment statistics indicate reluctance to embrace modern tools to advance British labs into the 21st century - Labmate Online - November 10th, 2019
- Human heart cells change during spaceflight, say scientists in study that could have far-reaching effects on c - MEAWW - November 7th, 2019
- Here's Why Fate Therapeutics Dropped as Much as 19.9% Today - Motley Fool - November 7th, 2019
- Space travel may change the human heart - Inverse - November 7th, 2019
- Allogene Therapeutics and Notch Therapeutics Announce Collaboration to Research and Develop Induced Pluripotent Stem Cell (iPSC)-Derived Allogeneic... - November 5th, 2019
- Goldfinch Bio to Present Oral and Poster Presentations at the American Society of Nephrology Kidney Week 2019 Annual Meeting - BioSpace - November 5th, 2019
- MD Anderson and Takeda Team Up on Next-Generation Immuno-Oncology Therapeutics - BioSpace - November 5th, 2019
- Induced Pluripotent Stem Cell Market is expected to witness a strong CAGR of 7.0% from 2018 to 2026 - Zebvo - November 2nd, 2019
- Can organoids, derived from stem cells, be used in disease treatments? - The Hindu - October 26th, 2019
- University team to seek approval for iPS-based heart treatment trial - The Japan Times - October 26th, 2019
- Global Gemcitabine Hydrochloride Market: Segmented By Application And Geography Trends, Growth And Forecasts To 2024 - Health News Office - October 26th, 2019
- Global consortium formed to combat unproven cell banking services - Drug Target Review - October 22nd, 2019
- Bloomberg Philanthropies, Johns Hopkins University School of Medicine, and The New York Stem Cell Foundation Research Institute Announce an... - October 22nd, 2019
- ISCT forms cell and gene therapy sector-wide coalition to combat the rise of unproven commercial cell banking services - PharmiWeb.com - October 21st, 2019
- Mutations Linked to Huntington's Increase Cells' Resistance to Manganese, Study Finds - Huntington's Disease News - October 15th, 2019
- Stem Cells Market : Insights Into the Competitive Scenario of the Market - Online News Guru - October 11th, 2019
- 'Rewind Therapeutics' and Remyelination - SciTech Europa - October 11th, 2019
- Cell Expansion Market is expected to rise at a remarkable CAGR during the Forecast Period 2016 2024 - Space Market Research - October 9th, 2019
- Global Induced Pluripotent Stem Cells Market 2019 Innovative Trends and Insights Research upto 2024 - News Adopt - October 5th, 2019
- New Rett Therapies May Stem From X-chromosome Reactivation Findings - Rett Syndrome News - October 5th, 2019
- Stemming the Tide of Alzheimer's - UCI News - October 5th, 2019
- Skin-Derived Heart Cells Help Uncover the Genetic Foundations of Cardiac Function - Technology Networks - October 5th, 2019
- IGIB finds a protein with better precision in gene-editing - The Hindu - October 5th, 2019
- Novel Cell Sorting and Separation Market: Focus on Acoustophoresis, Buoyancy-activated, Dielectrophoresis, Magnetophoretics, Microfluidics,... - October 5th, 2019
- Fabry Heart Cells Grown in Lab Dish Give Hints to Cardiac Complications - Fabry Disease News - September 27th, 2019
- Global Induced Pluripotent Stem Cells (iPSCs) Market 2019 Analysis & Forecast Report 2024 - Analytics News - September 24th, 2019
- Gene regulators work together for oversized impact on schizophrenia risk - National Institutes of Health - September 24th, 2019
- Blood Pressure and Prostate Treatment May Prevent or Slow Parkinson's, Early Study Suggests - Parkinson's News Today - September 24th, 2019
- New Gene Editing Technique Shown to Correct COL7A1 Gene in RDEB Cells - Epidermolysis Bullosa News - September 24th, 2019
- Vaginitis Therapeutics Market Overview with Detailed Analysis, Competitive lands - News By ReportsGO - September 24th, 2019
- Efavirenz/Tenofovir/Emtricitabine Combination Drug Market Overview with Detailed - News by Intelligence Journal - September 24th, 2019
- Can Consciousness be Created? - University Observer Online - September 24th, 2019
- Global Induced Pluripotent Stem Cells (iPSCs) Market 2019 Industry Growth with CAGR 12.7% in Forecast to 2024 - Indian Columnist - September 21st, 2019
- Adipose Tissue-derived Stem Cells Market Size Set for Rapid Growth and Trend by2018 2028 - My Health Reporter - September 21st, 2019
- Induced Pluripotent Stem Cell Market Estimated to be Driven by Innovation and Industrialization - Analytics News - September 20th, 2019
- 2024 Projections: Induced Pluripotent Stem Cells (iPSCs) Market Report by Type, Application and Regional Outlook - TheSlapClap - September 20th, 2019
- Angelman Foundation Honors 4 for Their Research, Advocacy Efforts - Angelman Syndrome News - September 20th, 2019
- Dicerna And DCR-A1AT In Alpha-1 Antitrypsin Deficiency-Associated Liver Disease - Seeking Alpha - September 20th, 2019
- Fate Therapeutics raises $173 million in offering - The San Diego Union-Tribune - September 19th, 2019
- Induced Pluripotent Stem Cells Market is expected to reach US$ 2299.5 Mn by the end of the forecast period in 2026 - Zebvo - September 19th, 2019
- induced pluripotent stem cells (iPSCs) market reached $2.1 billion in 2016 The market should reach $3.6 billion in 2021 - ScoopJunction - September 19th, 2019
- Stem Cell-Derived Cells Market to Record an Exponential CAGR by 2025 - NewsVarsity - September 19th, 2019
- Common Prostate Drug May Slow Progression of Parkinson, Researchers Say - AJMC.com Managed Markets Network - September 19th, 2019
- Automated Large-Scale Production of Retinal Organoids - Advanced Science News - September 19th, 2019
- How a Centuries-Old Sculpting Method Is Helping 3D Print Organs With Blood Vessels - Singularity Hub - September 19th, 2019
- Study indicates early infusion of mononuclear cells could aid in recovery from stroke - Yahoo Finance - September 19th, 2019
- Scientists recognize genes as master regulators in schizophrenia - Tech Explorist - September 19th, 2019
- Japanese lab to collaborate with Christian Dior in iPS cell research - Japan Today - September 19th, 2019
- Induced Pluripotent Stem Cell Market Research Report 2019 From TBRC has Been Updated - Market Research Gazette - September 19th, 2019
- What are Induced Pluripotent Stem (IPS) Cells? - Stem Cell ... - June 5th, 2019
- Induced Pluripotent Stem Cell - ScienceDirect - June 5th, 2019
- Stem Cell Glossary A Closer Look at Stem Cells - June 2nd, 2019
- Stemming retinal regeneration with pluripotent stem cells ... - May 27th, 2019
- Current Strategies and Challenges for Purification of ... - May 27th, 2019
- IPSCjun19 Induced Pluripotent Stem Cells: differentiation ... - May 24th, 2019
- Induced pluripotent stem cells don't increase genetic ... - April 30th, 2019
- Induced Pluripotent Stem Cells - Embryo Project - April 25th, 2019
- What Are Induced Pluripotent Stem Cells? - Stem Cell: The ... - April 13th, 2019
- Gene therapy-corrected autologous hepatocyte-like cells ... - April 3rd, 2019
- Regenerative Potential Of Induced Pluripotent Stem Cells ... - March 28th, 2019
- Induced Pluripotent Stem Cell (iPSC) Media and Reagents for ... - March 21st, 2019
- Embryonic or Induced Pluripotent Stem Cell Markers: R&D Systems - March 11th, 2019
- Differentiation of human induced pluripotent stem cells into ... - March 8th, 2019
- Do You Know the 5 Types of Stem Cells? | BioInformant - March 4th, 2019
- Global transcriptome analysis of pig induced pluripotent ... - March 4th, 2019
- Hypoimmunogenic derivatives of induced pluripotent stem ... - February 20th, 2019
- microRNA-690 regulates induced pluripotent stem cells (iPSCs ... - February 17th, 2019
- Induced pluripotent stem cells have been generated for the ... - February 17th, 2019
- Induction of Human Embryonic and Induced Pluripotent Stem ... - February 17th, 2019
- Pluripotent stem cells for improved reprogrammed Human ... - February 3rd, 2019
- Induced Pluripotent Stem Cell Market Is Expected to Reach US ... - February 3rd, 2019
- Pluripotent Stem Cell Flow Kit (FMC001): R&D Systems - February 3rd, 2019
- induced pluripotent stem cell | The Science of Parkinson's - February 3rd, 2019
- What Are Induced Pluripotent Stem Cells? | Intro to the ... - January 26th, 2019
- Pluripotent Stem Cells - Stemcell Technologies - December 30th, 2018
- ATCC-HYR0103 Human Induced Pluripotent Stem (IPS) Cells ATCC ... - December 30th, 2018
- Induced Pluripotent Stem Cells Market Report Research ... - December 30th, 2018
- Methods of Producing Thymic Emigrants from Induced ... - December 24th, 2018
- Global Induced Pluripotent Stem Cells Market Analysis (2018 ... - December 24th, 2018
- Human being induced pluripotent stem cells (hiPSCs) display ... - December 24th, 2018